[Chimera-users] volume viewer question

Tom Goddard goddard at sonic.net
Wed Aug 25 09:41:18 PDT 2010

Hi Boaz,

   That approach works too.  Getting the exact threshold isn't really so 
hard using my method though.  If using menu entry Measure Mean, SD, RMS 
reports RMS as 5.3 and I want the contour level to be 2 sigma, then I 
just type in 10.6 (= 2 * 5.3) in the volume dialog threshold level entry 
field and press the Enter key.


> Hi William,
> The way I go about the sigma level contouring in Chimera, though it's 
> somewhat ham-fisted but perhaps less complicated than Tom's 
> suggestion, is to contour the region of interest at the desired sigma 
> level in Coot or O and then play with the contouring in Chimera until 
> both maps look as similar as possible (the maps rarely look identical 
> for technical differences in the contouring techniques, I think). It 
> works.
>    Cheers,
>                Boaz

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