[Chimera-users] Movie Question: EM Densities about robbon structures

[Jared Godar]

Hello there,

I have a few technical questions about a movie I am trying to make with
chimera.

I have PDB files for three predicted structural elements of a larger protein
whose structure my lab is resolving through cryo EM.  I've converted these
PDB files to MRC's of the desired resolution we are trying to obtain with
the reconstruction.  In an effort to produce animation which highlights
structural elements of these three subdomains, I have encountered a couple
difficulties translation the movie I envision through Chimera.

I would like to start with the three subunits spaced across the X axis and
rotating about the central y axis of each subunit. Then, one at a time, have
a single unit grow and translate to the center, then while the other two
subunits fade away, then return to it's original position.

I've been able to script this type of motion for each of the individual
subunits, but I hit a problem when I try to incorporate all three.

   - Is there a way to have multiple proteins roll about their own
   respective y axis instead of orbiting about the central y axis of the scene?
   - Is there a way to selectively show / hide (fade over specified
   number of frames) PDB's or MRC's like you can show / hide / fade text?

Thank you in advance for your help.

-Jared

-- 
Jared A. Godar
Graduate Student: Stewart Lab
Vanderbilt University Medical Center
Molecular Physiology & Biophysics Dept.
Light Hall 708
Work: 615.322.7898
Fax:   615.322.7236
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