[chimera-dev] using chimera to display tomography map

Wei Zhang zhangwei at umn.edu
Wed Jan 1 08:40:30 PST 2020


Hello,

Happy 2020!

We are doing tomography reconstruction of retrovirus assemblies. Because
the raw tomograms are very noisy, they can not be represented nicely using
isosurfaces. I see other researchers in the field presented the tomo map in
Chimera by superimposing subtomogram-averaged map onto the original
tomogram according to the subunit's locations and  relative orientations.
The color of the displayed subunits is based on the correlation coefficient
between the averaged subunit map and the original density of the tomogram.

One example is Fig.1B in this paper:
https://advances.sciencemag.org/content/5/11/eaaw3631

We do use the program Dynamo for the subtomogram averaging procedure, so
our data format is very similar to what was used in this paper. Is this
feature is part of Chimera? Could we obtain the extended software? Or is
this customer built and we need to contact the authors of the paper? Or
shall we work out this procedure by ourselves with the help of Chimera
experts?

Thank you,

Wei
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